Higher fasting triglyceride predicts higher risks of diabetes mortality in US adults

Background It is unknown whether higher triglyceride results in higher mortality from diabetes, i.e., diabetes mortality. This study aimed to investigate the association of fasting triglyceride with diabetes mortality. Methods This study included 26,582 US adults from the National Health and Nutrition Examination Surveys from 1988 to 2014. Diabetes mortality outcomes were ascertained by linkage to the National Death Index records. Cox proportional hazards models were used to estimate hazard ratios (HRs) and 95% confidence intervals (CIs) of triglyceride for diabetes mortality. Results Higher levels of fasting triglyceride were associated with higher levels of glucose, glycated hemoglobin, insulin, and homeostatic model assessment for insulin resistance at baseline. A 1-natural-log-unit increase in triglyceride (e.g., from 70 to 190 mg/dL) was associated with a 115% higher multivariate-adjusted risk of diabetes diagnosis (odds ratio, 2.15; 95% CI, 2.00–2.33). During 319,758 person-years of follow-up with a mean follow-up of 12.0 years, 582 diabetes deaths were documented. Compared with people with triglyceride in the lowest quintile, people with triglyceride in the highest quintile had an 85% higher risk of diabetes mortality (HR, 1.85; 95% CI, 1.25–2.73). A 1-natural-log-unit increase in triglyceride was associated with a 40% higher multivariate-adjusted risk of diabetes mortality. The positive association between triglyceride and diabetes mortality was also presented in sub-cohorts of participants with or without diabetes. Conclusions This study demonstrated that higher fasting triglyceride was associated with a higher diabetes mortality risk. Supplementary Information The online version contains supplementary material available at 10.1186/s12944-021-01614-6.


Introduction
About 34.1 million adults-or 13.0% of all US adultshave diabetes, the seventh leading cause of death in the United States with a crude death rate of 25.7 per 100,000 persons per year [1]. The total direct and indirect estimated costs of diagnosed diabetes in the United States in 2017 alone were $327 billion [1]. Therefore, it is of great importance to identify modifiable factors to decrease diabetes incidence and diabetes-induced death, i.e., diabetes mortality.
Epidemiological studies frequently show that circulating triglyceride is higher in people with diabetes than that in those without diabetes [2,3]. Higher baseline triglyceride was reported to be associated with new-onset of diabetes in various populations such as Chinese [4], Japanese [5], and Americans [6]. These studies support the notion that higher triglyceride could lead to diabetes.
However, it is unknown whether higher circulating triglyceride leads to higher diabetes mortality. This study aimed to investigate whether higher fasting triglyceride at baseline predicted diabetes mortality using US adults who attended the National Health and Nutrition Examination Survey (NHANES) from 1988 to 2014.

Study participants
This study included participants from NHANES III (1988)(1989)(1990)(1991)(1992)(1993)(1994) and the subsequent eight cycles of NHAN ES from 1999 to 2014. The inclusion criteria included age of ≥20 years and presence of the fasting triglyceride data. This resulted in a cohort of 27,067 participants. The following participants were excluded: those without a follow-up time or with a follow-up time of 0 month (N = 31), and those who did not have data on the following parameters: plasma glucose (N = 57), blood glycated hemoglobin (HbA 1c , N = 70), serum insulin (N = 239), physical activity (N = 11), and education status (N = 77). Therefore, a total of 26,582 participants were included in the final analysis.

Fasting triglyceride
Fasting status was defined as a fasting time between 8.0 and 23.9 h. The concentration of fasting triglyceride in the serum was retrieved from the NHANES website. Triglyceride was measured enzymatically by using a series of coupled reactions in which triglyceride was hydrolyzed to produce glycerol [7]. Glycerol was then phosphorylated and oxidized to produce H 2 O 2 , and the latter, in the presence of peroxidase, produced a color product that was measured spectrophotometrically at the wavelength of 500 nm. Triglyceride was treated as a continuous variable (natural log-transformed) or a categorical variable (quintiles) in the analysis.

Diabetes-related markers and definition of diabetes
The following diabetes-related markers were retrieved from the NHANES website: plasma glucose, HbA 1c in the whole blood, and serum insulin. Homeostatic model assessment (HOMA) for insulin resistance was calculated using the following formula [8]: (serum insulin in μU/mL X plasma glucose in mmol/L)/22.5.

Diabetes mortality
Data on diabetes-caused mortality were directly retrieved from NHANES-linked mortality files. To evaluate mortality status and the cause of death, the National Center for Health Statistics conducted probabilistic matching to link the NHANES data with death certificate records from the National Death Index records. The NHANESlinked mortality files used the Underlying Cause of Death 113 (UCOD_113) code to recode all deaths according to the International Classification of Diseases, 9th Revision (ICD-9) or the International Classification of Diseases, 10th Revision (ICD-10). Diabetes mortality was defined as diabetes being listed as an underlying cause of death. Follow-up time was defined as the time (in months) from the time when the blood was drawn at the Mobile Examination Center until death, or until the end of follow-up (i.e., December 31, 2015), whichever occurred first.

Statistical analyses
Data were presented as mean and standard deviation for continuous variables or percentages for categorical variables. Difference in age was analyzed using Student's ttest and differences in non-normally distributed continuous variables (triglyceride, glucose, HbA 1c , insulin, HOMA for insulin resistance, and C-reactive protein) were analyzed using the Mann-Whitney U test between those with or without diabetes. Differences among categorical variables were analyzed using Pearson's chisquare test. Associations of triglyceride with diabetesrelated markers (glucose, HbA 1c , insulin, and HOMA for insulin resistance) were assessed using the least-squares regression [22], with or without adjustment for confounding factors including age, sex, ethnicity, obesity, education, income, lifestyle factors (physical activity, alcohol consumption, and smoking status), survey period, and clinical confounders (hypercholesterolemia, hypertension, diabetes, and family history of diabetes). Triglyceride, glucose, HbA 1c , insulin, and HOMA for insulin resistance were natural log-transformed to improve the data distribution before being put into the models. Binary logistic regression was used to assess the association of triglyceride with diabetes diagnosis, with or without adjustment for the above confounders [23]. Cox proportional hazards models were used to calculate hazard ratios (HRs) and 95% confidence intervals (CIs) of triglyceride for diabetes mortality. Sub-analyses were conducted in sub-cohorts of participants with or without diabetes.
Sensitivity analyses were conducted by further adjustment for baseline glucose, or high-density lipoprotein (HDL) cholesterol and low-density lipoprotein (LDL) cholesterol, as higher levels of glucose [11], lower levels of HDL cholesterol [24], and higher levels of LDL cholesterol [25,26] were associated with new-onset of diabetes. Sensitivity analyses were also conducted by further adjustment for C-reactive protein [27] or the use of lipid-lowering drugs. In addition, sensitivity analyses were conducted when 2-h post-load glucose was considered as a criterium for diabetes diagnosis or when diabetes mortality was defined as diabetes being listed as the leading cause of death.
The null hypothesis was rejected for two-sided values of P < 0.05. All analyses were performed using SPSS version 27.0 (IBM SPSS Statistics for Windows, Armonk, NY, IBM Corporation).

General characteristics
This study included 26,582 (22,909 without diabetes and 3673 with diabetes) US adults with a mean (SD) age of 49 (19) years. Baseline characteristics of the participants are displayed in Table 1.

Association of circulating triglyceride with diabetesrelated markers
Higher triglyceride was independently associated with higher plasma glucose, higher HbA 1c , higher serum insulin, and higher HOMA for insulin resistance in the whole cohort and sub-cohorts of participants with or without diabetes ( Table 2).

Association of circulating triglyceride with diabetes mortality
During 319,758 person-years of follow-up with a mean follow-up of 12.0 years, 582 participants died from diabetes. A 1-natural-log-unit increase in triglyceride was associated with a 40, 32, and 45% higher multivariateadjusted risk of diabetes mortality in the whole cohort, and sub-cohorts of participants with or without diabetes, respectively (Table 4).

Sensitivity analyses
Sensitivity analyses showed that the association of triglyceride with diabetes mortality was attenuated but remained significant after further adjustment for baseline plasma glucose (Supporting Information Table S1), or HDL and LDL cholesterol (Supporting Information  Table S2). Higher triglyceride was associated with higher serum C-reactive protein (Supporting Information Table  S3), and further adjustment for serum C-reactive protein did not materially change the association between fasting triglyceride and diabetes mortality (Supporting Information Table S4). The significance of the results remained after further adjustment for use of lipid-lowering drugs (Supporting Information Table S5), or when 2-h postload glucose of ≥200 mg/dL was considered as another criterium for diabetes diagnosis (Supporting Information  Tables S6-7), or when diabetes mortality was defined as diabetes being listed as the leading cause of death (Supporting Information Table S8).

Discussion
Using a representative cohort of US adults, this study, for the first time, revealed that higher fasting triglyceride at baseline was associated with a higher risk of diabetes mortality. Such an association was also presented in subcohorts of participants with or without diabetes.
The finding that higher circulating triglyceride predicted higher diabetes mortality is supported by the following findings of the current study: 1) higher triglyceride was associated with higher plasma glucose, blood HbA 1c , serum insulin, and HOMA for insulin resistance; 2) higher triglyceride was associated with higher C-reactive protein, and the latter is an inflammatory marker and an independent predictor for diabetes [27]; and 3) higher triglyceride was associated with a higher risk of diabetes diagnosis. The mechanism(s) underlying the association between higher triglyceride and higher diabetes mortality is not clear. One possible mechanism could be due to triglyceride-induced inflammation [28] which was supported by the observation of the current study that higher triglyceride was associated with higher levels of C-reactive protein in the circulation. However, the sensitivity analyses showed that further adjustment for Creactive protein did not attenuate the association between triglyceride and diabetes mortality, suggesting that the association between higher triglyceride and higher diabetes mortality may not be mediated by C-reactive protein (or inflammation). Another possible mechanism could be that the change in triglyceride may be copresented with changes in HDL and LDL cholesterol [29]. Sensitivity analyses showed that further adjustment for HDL and LDL cholesterol did attenuate but did not abolish the association between triglyceride and diabetes mortality. Baseline glucose was a strong predictor of future diabetes [27]. Future adjustment for baseline plasma glucose did attenuate but did not abolish the association between triglyceride and diabetes mortality. All these results suggest that circulating triglyceride was an independent risk factor for future diabetes mortality, and the association may be partially mediated by plasma glucose, and HDL and LDL cholesterol.
Epidemiological [2,3] and longitudinal cohort studies [4-6, 30, 31] showed that higher triglyceride may be a cause of new-onset of diabetes. However, this is not without controversy, as genetic studies provided mixed results. For example, triglyceride-increasing alleles have been reported to be either not associated with [32], or  positively associated with [33], or inversely associated with diabetes [6,34,35]. One of the genetic studies [6] revealed inconsistency between genetic and non-genetic results from the same cohort: triglyceride-increasing alleles were associated with lower new-onset of diabetes, whereas higher baseline circulating levels of triglyceride were associated with higher new-onset of the disease. The reason underlying the observed inconsistency [6] is unknown; it might be due to the selection of genetic alleles or due to that some of the selected genetic alleles have other functions beyond regulating circulating triglyceride.
In people without diabetes at baseline, higher fasting triglyceride predicted impaired glucose tolerance [36], impaired fasting glucose (IFG), and new-onset of diabetes [4-6, 29, 30]. The results of the current study showed that, in participants without diabetes at baseline, higher fasting triglyceride predicted diabetes mortality, supporting the notion that higher triglyceride may be a cause rather than an effect of diabetes.
It has been reported that higher triglyceride was associated with higher all-cause mortality [37,38]. The current study suggested that the positive association between triglyceride and all-cause mortality may be mediated, at least in part, by higher diabetes mortality. Diabetes can lead to increased risks for cardiovascular disease including heart failure, and glycemic control drugs including sodium-glucose transporter 2 (SGLT2) inhibitors have substantially improved the management of patients with diabetes and reduced patients' cardiovascular complications [39]. Higher fasting triglyceride has been shown to be associated with higher cardiovascular disease mortality [40]. Therefore, reduced cardiovascular disease mortality might be involved in the reduced all-cause mortality associated with lower triglyceride.

Comparisons with other studies and what does the current work add to the existing knowledge
Although it has been widely reported that higher triglyceride is associated with diabetes prevalence and incidence [2-6, 30, 31], one important piece of information is missing from the literature, i.e., whether higher triglyceride is associated with higher diabetes mortality. The current study filled this knowledge gap. Diabetes mortality could be defined as diabetes being listed as an underlying cause of death or as the leading cause of death. In either case, higher triglyceride was found to be associated with higher diabetes mortality in the general US population.

Strengths and limitations
A strength of this study is its large sample size (N = 26,582). This study has several other strengths, such as a prospective study design, the use of a nationally representative sample of US adults with or without diabetes, and adjustment for a large number of confounding factors. This study also has a number of limitations. First, triglyceride was only measured at one time point, which may result in misclassification. Nevertheless, this misclassification tends to result in an underestimate rather than an overestimate of risk in epidemiological analysis due to the effect of regression dilution bias [41]. Second, mortality outcomes were ascertained by linkage to the National Death Index (NDI) records with a probabilistic match, which may also lead to misclassification. However, the Special Projects Branch from the National Center for Health Statistics employed a matching methodology offered by the NDI to determine the best    Fig. 1 Summary of the study. In the cross-sectional analyses, higher triglyceride was associated with higher levels of diabetes markers and higher risks for diabetes diagnosis. In the longitudinal analyses, higher triglyceride predicted higher diabetes mortality, which might be partially mediated by PG, HDL cholesterol, and LDL cholesterol, but not by C-reactive protein. ↑, increased; DM, diabetes; HbA1c, glycated hemoglobin; HDL-C, high-density lipoprotein cholesterol; HOMA-IR, homeostatic model assessment for insulin resistance; LDL-C, low-density lipoprotein cholesterol; PG, plasma glucose match [42] and a prior validation study showed that the matching method had high accuracy (98.5%) [43].

Conclusion
This study demonstrated that higher fasting triglyceride was associated with a higher risk of diabetes diagnosis and diabetes mortality (Fig. 1). Triglyceride may need to be routinely monitored in clinic for the risk assessment for diabetes. In addition, triglyceride may be a therapeutic target for the management of diabetes, and clinical trials on the effectiveness of lowering triglyceride to prevent diabetes mortality are needed.